The image data used in the challenge was produced in Lichtman Lab at Harvard University (Daniel R. Berger, Richard Schalek, Narayanan "Bobby" Kasthuri, Juan-Carlos Tapia, Kenneth Hayworth, Jeff W. Lichtman) and manually annotated by Daniel R. Berger. Their corresponding biological findings were published in Cell (2015).

The organizers thank Alessandro Guisti, Dan Ciresan, Luca M. Gambardella and Juergen Schmidhuber from the Swiss AI Lab IDSIA (Istituto Dalle Molle di Studi sull'Intelligenza Artificiale) for providing the 2D membrane probabilities of the challenge data sets.